Antioxidative Activity and Nitrite Scavenging Ability of Ethanol Extract from Nelumbo nucifera Leaves
To confirm possibility of Nelumbo nucifera leaves as biofunctional material, we investigated the antioxidant activity and nitrite scavenging abilty of ethanol extract from Nelumbo nucifera leaves. Nelumbo nucifera leaves were extracted with ethanol and concentrated under vacuum using rotary evaporator. Then, antioxidative activity and nitrite scavenging ability of the extract were examined in vitro. Electron -donating ability of the extract at RC50 was 90.19 ㎍/mL. After addition of 0.96 mg/mL, autooxidation of pyrogallol decreased to 66.19% by superoxide dismutase-like activity. In peroxidation values significantly decreased to 72.53%, 82.00%, 84.69% with addition of 0.2 mg/mL, respectively. Total phenolic content was determined as gallic acid equivalents (GAE) and the value revealed to be 282.84 ± 9.03% GAE ㎍/mg of the extract. Nitrite scavenging ability showed the most remarkable effect at pH 1.2, exhibited to 45.55% by addition of 0.2 mg/mL. These results suggest that ethanol extract from N. nucifera leaves can be used as bioactive and functional material.
Jin A Lim and Eun Sook Lee and Seung Hwa Baek, “Antioxidative Activity and Nitrite Scavenging Ability of Ethanol Extract from Nelumbo nuciefra Leaves”, Korean J. Oriental Physiology & Pathology 22(3);p654~659, 2008